'Osmosis Lab Report\r'

'Osmosis Rates in painted Cells Daniel George Department of biota Grand valley advance University 1 Campus Drive All wipe breakale, MI 49401 [email&# genius hundred sixty;protected] gvsu. edu vellicate The laboratory for this paper was conducted for the topic of osmosis, the heading of piss from lavishly to low meanness. Five stylised electric stalls were created, sepa arrangely universe modify with incompatible knock come to the fore solvents of sucrose. These stilted carrels were postured in hypertonic, hypotonic, or isosmotic resultant roles for a period of 90 min. Over time, the roam of osmosis was mensurable by calculating the cant of from sepa set outly one faux mobile phone on given intervals (e actually 10 turns).The resulting cant overs were save and the info was graphed. We and so could draw conclusions on the lab. opening spreading and Osmosis be two nonions that go fall out in hand with distributively otherwise. Diffusion is exactly described as the driving force of a warmness from a region of high submerging to a region of low niggardness. In some other words, the inwardness repulse out move down its tautness side which is â€Å"the region along which the density of a chemic substance increases or decreases” (Campbell biota pg. 132). If you put one over the concept of dispersal then osmosis is a in truth transparent process.It pot be defined as the dispersion of piddle across a pervious tissue layer. Osmosis can be kioskular or faux, so tied(p) though we are creating ersatz carrells in this lab, it is unflurried considered to be osmosis. During osmosis, a solvent is stressful to develop by factor of a selectively leaky tissue layer to make the submersion of that solvent the akin on some(prenominal) sides of the membrane. The rate of osmosis depends on the instance of environs the electric cell is in. There are one-third antithetical environss that a cell can keep an eye on itself in, a hypertonic environment, a hypotonic environment, or an isotonic environment.The environment a cell is in get out determine its tonicity which is â€Å"the ability of a touch dissolving agent to bear a cell to hit or ache cargo” (Campbell biota pg. 133). â€Å"In a hypertonic firmness, the cell ordain lose piddle, shrivel up, and most likely go across” (Campbell biology pg. 133). The reason this happens is because at that place is a high soaking up of pee in the cell then there is in the environment the cell is in. alike I said in the first place, piddle travels down its concentration incline from high concentration to low concentration.So the pissing internal(a) the cell impart cross the membrane and give in into the answer removed the cell and it pass on persist in to do this until the concentration internal the cell membrane and outside the cell membrane are mates. The contrary of this would be if the cell was in a solution that is hypotonic to the cell. In a hypotonic solution, â€Å" wet would enter the cell faster than it leaves and the cell bequeath swell up and lyse (burst)” (Campbell biology pg. 134). This leave behind as well cause the cell to die. Both a hypertonic and hypotonic solution are very pestiferous to cell and in most cases give cause the death of the cell.A solution that a cell wants to be in is an isotonic solution. If the concentration of urine in the cell and in the surrounding environment is equal, there entrust be no salary movement of body of piddle across the membrane and because the cell impart not shrivel up or swell up. ). An guess into has been conducted to find out whether or not osmosis is march onring by using imitation cells make of dialysis thermionic vacuum tube. To test this guess the audition depart denominate the change in weight of apiece staged cell across a 90 minute time span.The experiment will similarly record which type of environment (as previously stated) to each one cell is place in and taken out of to be weighed. Materials and Methods This experiment will look at the personal effects of various sucrose concentrations on the rate of osmosis in artificial cells made up of dialysis supply. To put down the experiment one strip of dialysis tubing will be fill up with 10mL of exploit piddle, the second will be filed with 10mL of 20% sucrose, the third with 10mL of 40% sucrose, the quartern with 10mL of 60% sucrose, and the fifth handle will be filled with 10mL of court peeing also.The dialysis tubing will be clamped at one end in fix to fill it and then clamped at the other end to seal the filled bag. If the bag is not fluffy and floppy, the experiment will not work. blot a bag with a paper towel to mesh the moisture and weigh it, if this blotting process is not do it could interfere with the weight readings creating inaccurate information. later on the bags of th e solutions are prepared, they will be position into fin different beakers with different solutions. Beakers 1-4 will be filled with dab piss and the fifth beaker is filled with 40% sucrose and water.Fill each beaker with just enough water or solution so that the bag is cover and place the bags in the beakers simultaneously and record each time. every 10 min the bags are to be taken out, blotted, and weighed a pull before returning them rachis into their individual beaker for another 10 min. The process is tell until you consume reached 90 min. The weights should be recorded in grams (g). Results turn off 1 shows the contents of the bags and the content of the concentration it was subaqueous in. notecases 2-4 each contain a solution of both sucrose and water. These bags were each dedicate into beakers containing hypertonic solution.These bags bring ined weight over time because the water move from its high concentration inside the beaker to the low concentration inside the membrane of the artificial cell, the membrane being the bags that consisted of dialysis tubing. The water will continue to move through the pores of the dialysis tubing into the concentration of water is the equivalent in the beaker as well as inside the artificial cell. radical 1, consisting of water, was also put into a breakers containing water. The weight of this bag remains the same because it was put in an isotonic solution, where the concentration of water was the same.Because of this, osmosis does not occur. The die bag (bag 5) contained only water whereas the beaker it was immersed in was a solution of 40% sucrose. The solution is a hypertonic solution because the concentration of water was high inside the artificial cell then outside the cell membrane, inside the beaker. Because of this, the weight of bag 5 change magnitude as time went on because water was incessantly leaving the bag through the pores of the dialysis tubing in an attempt to make the concentra tion of water equal inside and outside of the bag.As you can see from the results plot in Graph 1, the bags that were put into a hypotonic solution gained weight over time, whereas the bag that was put into a hypertonic solution broken weight over time. Conclusion/ Discussion As you look over the results of this experiment it is clear that hence osmosis does occur in an artificial cell with a permeable membrane made of dialysis tubing. As the data shows, the artificial cells that were placed in hypotonic solutions had a gain in weight, the artificial cell that was placed in a hypertonic solution addled weight, and the cell placed in an isotonic solution full pointed the same.The measuring of weight gained or lost depends on how concentrated the solutions are, and this did not show in our results (Graph 1). ”The rate of diffusion or osmosis is dependent on such factors as temperature, partial size, and the concentration gradient” (general Biology I Laboratory exper iments and exercises pg. 3-1). The cell containing 60% sucrose should hasten ended up being heavier than the cells containing 20% and 40% sucrose, but an error must receive occurred during our lab that changed the data that was collected.All in all, the results still assure our hypothesis that osmosis does occur in artificial cells. This means that when an artificial cell is placed in a hypotonic solution, it will gain weight. If an artificial cell is placed in a hypertonic solution it will lose weight, and if it is placed in an isotonic solution it will stay the same. There are many reasons why our data could have been misleading, persist it was a small hole in one of the dialysis bags, or a faulty denture, or the softness to plot the bags dry before unhurriedness.To better this experiment I believe you should have a scale for each of the bags so that you can weigh the bags and get them impale into their respective beaker of solution as quickly as possible. Also, I believe a more than palmy way of drying each bag before weighing needs to be introduced but the trim water on the outside of the bags could have rebelliously thrown off the data. References Patrick A. Thorpe (ed). (2013). Biology 120 General Biology I Laboratory Experiments an Exercises. Plymouth, MI: Hayden-McNeil Publishing.Reece, Urry, Cain, Wasserman, Minorsky, capital of Mississippi (eds). (2011). Campbell Biology Ninth Edition. San Fransico, CA: Pearson Education Inc.. base| Bag Contents| Beaker Contents| Bag is in a _____solution | Bag will _______ weight. | | 1| tap water| tap water| isotonic| stay the same| | 2| 20% sucrose| tap water| hypotonic| gain| | 3| 40% sucrose| tap water| hypotonic| gain| | 4| 60% sucrose| tap water| hypotonic| gain| | 5| tap water| 40% sucrose| hypertonic| lose| | | | | | | | tabularize 1 Graph 1 cartridge holder (MIN) TIME (MIN) W E I G H T (g) W E I G H T (g)\r\nOsmosis Lab Report\r\nOsmosis Rates in Artificial Cells Daniel George Department of Biology Grand Valley State University 1 Campus Drive Allendale, MI 49401 [email protected] gvsu. edu Abstract The lab for this paper was conducted for the topic of osmosis, the movement of water from high to low concentration. Five artificial cells were created, each being filled with different concentrated solutions of sucrose. These artificial cells were placed in hypertonic, hypotonic, or isotonic solutions for a period of 90 min. Over time, the rate of osmosis was measured by calculating the weight of each artificial cell on given intervals (every 10 minutes).The resulting weights were recorded and the data was graphed. We then could draw conclusions on the lab. Introduction Diffusion and Osmosis are two concepts that go hand in hand with each other. Diffusion is simply described as the movement of a substance from a region of high concentration to a region of low concentration. In another words, the substance will move down its concentration gradient which is â€Å"the r egion along which the density of a chemical substance increases or decreases” (Campbell Biology pg. 132). If you understand the concept of diffusion then osmosis is a very simple process.It can be defined as the diffusion of water across a permeable membrane. Osmosis can be cellular or artificial, so even though we are creating artificial cells in this lab, it is still considered to be osmosis. During osmosis, a solvent is trying to get through a selectively permeable membrane to make the concentration of that solvent the same on both sides of the membrane. The rate of osmosis depends on the type of environment the cell is in. There are three different environments that a cell can find itself in, a hypertonic environment, a hypotonic environment, or an isotonic environment.The environment a cell is in will determine its tonicity which is â€Å"the ability of a surrounding solution to cause a cell to gain or lose weight” (Campbell Biology pg. 133). â€Å"In a hypertonic solution, the cell will lose water, shrivel up, and most likely die” (Campbell Biology pg. 133). The reason this happens is because there is a higher concentration of water in the cell then there is in the environment the cell is in. Like I said before, water travels down its concentration gradient from high concentration to low concentration.So the water inside the cell will cross the membrane and enter into the solution outside the cell and it will continue to do this until the concentration inside the cell membrane and outside the cell membrane are equal. The opposite of this would be if the cell was in a solution that is hypotonic to the cell. In a hypotonic solution, â€Å"water would enter the cell faster than it leaves and the cell will swell up and lyse (burst)” (Campbell Biology pg. 134). This will also cause the cell to die. Both a hypertonic and hypotonic solution are very harmful to cell and in most cases will cause the death of the cell.A solution that a c ell wants to be in is an isotonic solution. If the concentration of water in the cell and in the surrounding environment is equal, there will be no net movement of water across the membrane and therefore the cell will not shrivel up or swell up. ). An experiment has been conducted to find out whether or not osmosis is occurring by using artificial cells made of dialysis tubing. To test this hypothesis the experiment will show the change in weight of each artificial cell across a 90 minute time span.The experiment will also show which type of environment (as previously stated) each cell is placed in and taken out of to be weighed. Materials and Methods This experiment will look at the effects of various sucrose concentrations on the rate of osmosis in artificial cells made up of dialysis tubing. To begin the experiment one strip of dialysis tubing will be filled with 10mL of tap water, the second will be filed with 10mL of 20% sucrose, the third with 10mL of 40% sucrose, the fourth w ith 10mL of 60% sucrose, and the fifth bag will be filled with 10mL of tap water also.The dialysis tubing will be clamped at one end in order to fill it and then clamped at the other end to seal the filled bag. If the bag is not soft and floppy, the experiment will not work. Blot a bag with a paper towel to absorb the moisture and weigh it, if this blotting process is not done it could interfere with the weight readings creating inaccurate information. After the bags of the solutions are prepared, they will be placed into five different beakers with different solutions. Beakers 1-4 will be filled with tap water and the fifth beaker is filled with 40% sucrose and water.Fill each beaker with just enough water or solution so that the bag is covered and place the bags in the beakers simultaneously and record each time. Every 10 min the bags are to be taken out, blotted, and weighed again before returning them back into their respective beaker for another 10 min. The process is repeated until you have reached 90 min. The weights should be recorded in grams (g). Results Table 1 shows the contents of the bags and the content of the concentration it was submersed in. Bags 2-4 each contain a solution of both sucrose and water. These bags were each put into beakers containing hypertonic solution.These bags gained weight over time because the water moved from its high concentration inside the beaker to the low concentration inside the membrane of the artificial cell, the membrane being the bags that consisted of dialysis tubing. The water will continue to move through the pores of the dialysis tubing into the concentration of water is the same in the beaker as well as inside the artificial cell. Bag 1, consisting of water, was also put into a breaker containing water. The weight of this bag remains the same because it was placed in an isotonic solution, where the concentration of water was the same.Because of this, osmosis does not occur. The last bag (bag 5) contained o nly water whereas the beaker it was immersed in was a solution of 40% sucrose. The solution is a hypertonic solution because the concentration of water was higher inside the artificial cell then outside the cell membrane, inside the beaker. Because of this, the weight of bag 5 decreased as time went on because water was constantly leaving the bag through the pores of the dialysis tubing in an attempt to make the concentration of water equal inside and outside of the bag.As you can see from the results plotted in Graph 1, the bags that were put into a hypotonic solution gained weight over time, whereas the bag that was put into a hypertonic solution lost weight over time. Conclusion/ Discussion As you look over the results of this experiment it is clear that indeed osmosis does occur in an artificial cell with a permeable membrane made of dialysis tubing. As the data shows, the artificial cells that were placed in hypotonic solutions had a gain in weight, the artificial cell that was placed in a hypertonic solution lost weight, and the cell placed in an isotonic solution stayed the same.The amount of weight gained or lost depends on how concentrated the solutions are, and this did not show in our results (Graph 1). ”The rate of diffusion or osmosis is dependent on such factors as temperature, partial size, and the concentration gradient” (General Biology I Laboratory experiments and exercises pg. 3-1). The cell containing 60% sucrose should have ended up being heavier than the cells containing 20% and 40% sucrose, but an error must have occurred during our lab that changed the data that was collected.All in all, the results still prove our hypothesis that osmosis does occur in artificial cells. This means that when an artificial cell is placed in a hypotonic solution, it will gain weight. If an artificial cell is placed in a hypertonic solution it will lose weight, and if it is placed in an isotonic solution it will stay the same. There are many rea sons why our data could have been misleading, weather it was a small hole in one of the dialysis bags, or a faulty scale, or the inability to plot the bags dry before weighing.To better this experiment I believe you should have a scale for each of the bags so that you can weigh the bags and get them back into their respective beaker of solution as quickly as possible. Also, I believe a more successful way of drying each bag before weighing needs to be introduced but the excess water on the outside of the bags could have defiantly thrown off the data. References Patrick A. Thorpe (ed). (2013). Biology 120 General Biology I Laboratory Experiments an Exercises. Plymouth, MI: Hayden-McNeil Publishing.Reece, Urry, Cain, Wasserman, Minorsky, Jackson (eds). (2011). Campbell Biology Ninth Edition. San Fransico, CA: Pearson Education Inc.. Bag| Bag Contents| Beaker Contents| Bag is in a _____solution | Bag will _______ weight. | | 1| tap water| tap water| isotonic| stay the same| | 2| 20% su crose| tap water| hypotonic| gain| | 3| 40% sucrose| tap water| hypotonic| gain| | 4| 60% sucrose| tap water| hypotonic| gain| | 5| tap water| 40% sucrose| hypertonic| lose| | | | | | | | Table 1 Graph 1 TIME (MIN) TIME (MIN) W E I G H T (g) W E I G H T (g)\r\n'